Plants live in tight associations with microbes who colonize their roots and leaves and surrounding soil. Some microbes are harmful and cause disease while others are beneficial and aid in plant nutrient uptake, decomposition of organic materials, protection against pathogens, tolerance against drought and other stressors. Plants can influence microbial communities and vice versa with consequences for the growth of individual plants, the composition of plant communities, and entire ecosystems. In our work, we focus on three main aspects of plant-microbe interactions; 1) how they may aid or hinder plant invasions, 2) how their function changes across environmental gradients, and 3) how they may both cause and prevent disease in plants.
On MPG Ranch, three highly invasive weeds of contrasting lie history strategies (cheatgrass, knapweed and leafy spurge) co-occur with remnants of native plant vegetation. Using both observational and experimental approaches, we seek to understand how these weeds alter microbial communities and how this influence invasive success, ecosystem properties and restoration. We also collaborate with researchers from across the world to learn if plant-microbe interactions differ between native and invasive ranges and how this correlates with evolutionary shifts in plant genomes and biogeographical distribution of plant associated microbes.
Outcomes of plant-soil microbe interactions depend on the particular plant and fungal species and surrounding environmental conditions. To explore this context-dependency, we use high-throughput sequencing and stable and radioactive isotopes in surveys and experiments to determine if the proportion of fungal guilds (mutualistic mycorrhizal fungi and potential pathogens) change with water and nutrient availability and how these changes relate to plant growth. Because most research occurs in single locations, the generality of findings across locations that differ in environmental conditions is often unknown. To address this, MPG Ranch is part of two global research collaborations, Nutrient Network (https://nutnet.org) and DroughtNet (www.drought-net.org). In these experiments, all researchers apply nutrients, remove herbivores, expose plants to drought, and record responses in both plant and microbial communities using the same protocols, which allow for direct comparisons across sites.
The invasive fungal pathogen, Cronartium ribicola, causes the disease commonly known as blister rust in all nine white pine species native to the United States. As one of the only labs in the world to grow C. ribicola in culture, we perform tests of pathogen metabolism when exposed to compounds produced by other fungi found in white pine needles. Greenhouse experiments inoculating trees with these fungi, as well as beneficial ectomycorrhizal fungi, explore how we can improve tree growth and disease resistance. We also use isotopes and controlled field experiments to determine how blue-stain fungi carried by bark beetles can influence wood decomposition in forest ecosystems. As warming climates increase the frequency of bark beetle outbreaks worldwide, this research will help to better estimate future forest carbon storage and release.
Min Sheng, Lorinda Bullington, Morgan McLeod, Ylva Lekberg (2022). Acquisition and evolution of enhanced mutualism—an underappreciated mechanism for invasive success? The ISME Journal https://doi.org/10.1038/s41396-022-01293-w.
Dean E. Pearson, Özkan Eren, Yvette K. Ortega, José L. Hierro, Birsen Karakuş, Sascha Kala, Lorinda Bullington, Ylva Lekberg (2022). Combining biogeographical approaches to advance invasion ecology and methodology. Journal of Ecology 10.1111/1365-2745.13945.
Gerard Sapes, Patrick Demaree, Ylva Lekberg, Anna Sala (2021). Plant carbohydrate depletion impairs water relations and spreads via ectomycorrhizal networks. New Phytologist 229: 3172-3183. https://doi.org/10.1111/nph.17134.
Ylva Lekberg, Carlos A. Arnillas, Elizabeth T. Borer, Lorinda S. Bullington, Noah Fierer, Peter G. Kennedy, Jonathan W. Leff, Angela D. Luis, Eric W. Seabloom, Jeremiah A. Henning (2021). Nitrogen and phosphorus fertilization consistently favor pathogenic over mutualistic fungi in grassland soils. Nature Communications 12, 3484 (2021). https://doi.org/10.1038/s41467-021-23605-y.
Mandy L. Slate, Morgan Luce McLeod, Ragan M. Callaway (2021). Positive interactions between an exotic invader and moss biocrusts vary across life stage and correspond with the effect of water pulses on soil nitrogen. Functional Ecology 00, 1– 11. https://doi.org/10.1111/1365-2435.13831.
Vasilis Kokkoris, Ylva Lekberg, Pedro M. Antunes, Catherine Fahey, James A. Fordyce, Stephanie N. Kivlin, Miranda M. Hart (2020). Codependency between plant and arbuscular mycorrhizal fungal communities: what is the evidence? New Phytologist 228: 828-838. https://doi.org/10.1111/nph.16676.
Bullington, L. S., Lekberg, Y., Sniezko, R., & Larkin, B. (2018). The influence of genetics, defensive chemistry and the fungal microbiome on disease outcome in whitebark pine trees. Molecular plant pathology 19(8), 1847-1858. https://doi.org/10.1111/mpp.12663.
Lekberg, Y., Bever, J. D., Bunn, R. A., Callaway, R. M., Hart, M. M., Kivlin, S. N., ... & Remke, M. (2018). Relative importance of competition and plant–soil feedback, their synergy, context dependency and implications for coexistence. Ecology Letters 21(8), 1268-1281. https://doi.org/10.1111/ele.13093.
Waller, L. P., Hahn, P. G., Maron, J. L., & Lekberg, Y. (2018). Trait differences in responses to arbuscular mycorrhizal fungi are stronger and more consistent than fixed differences among populations of Asclepias speciosa. American Journal of Botany 105(2), 207-214. https://doi.org/10.1002/ajb2.1038.
Hahn, P. G., Bullington, L., Larkin, B., LaFlamme, K., Maron, J., & Lekberg, Y. (2018). Effects of Short-and Long-Term Variation in Resource Conditions on Soil Fungal Communities and Plant Responses to Soil Biota. Frontiers in Plant Science 9, 1605. https://doi.org/10.3389/fpls.2018.01937.
Hahn, P. G., Bullington, L., Larkin, B., LaFlamme, K., Maron, J. L., & Lekberg, Y. (2018). Effects of short-and long-term variation in resource conditions on soil fungal communities and plant responses to soil biota. Frontiers in Plant Science 9, 1605. https://doi.org/10.3389/fpls.2018.01605.
McLeod, M. L., Rummel, A., & Lekberg, Y. (2018). A rapid, quantitative, and affordable fluorometric method to determine the viability of roots with potential application in fungal hyphae. Rhizosphere 6, 1-6. https://doi.org/10.1016/j.rhisph.2018.01.008.
Lekberg, Y., Wagner, V., Rummel, A., McLeod, M., & Ramsey, P. W. (2017). Strong indirect herbicide effects on mycorrhizal associations through plant community shfts and secondary invasions. Ecological Applications 27(8), 2359-2368. https://doi.org/10.1002/eap.1613.
New Phytologist (2017). Historical biome distribution and recent human disturbance shape the diversity of arbuscular mycorrhizal fungi. Partel, M., Opik, M., Moora, M., Tedersoo, L., Szava-Kovats, R., Rosendahl, S., Rillig, M. C., Lekberg, Y., Kreft, H., Helgason, T., Eriksson, O., Davison, J., deBello, F., Caruso, T., & Zobel, M. 216(1), 227-238. https://doi.org/10.1111/nph.14695.
Reinhart, K. O., Lekberg, Y., Klironomos, J., & Maherali, H. (2017). Does responsiveness to arbuscular mycorrhizal fungi depend on plant invasive status? Ecology and Evolution 7(16), 6482-6492. doi: 10.1002/ece3.3226.
Bennett, J. A., Maherali, H., Reinhart, K. O., Lekberg, Y., Hart, M. M., & Klironomos, J. (2017). Plant-soil feedbacks and mycorrhizal type influence temperate forest population dynamics. Science 355(6321), 181-184. doi: 10.1126/science.aai8212.